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Title: Halophilic and Halotolerant Plant Growth Promoting Rhizobacteria (PGPR) from the Rhizosphere of Tamarix aucheriana in Kuwait
Authors: Dalal Abdulrahman Al-Bannai 
Supervisor: Dr. Nedaa Y. Ali Alsarraf
Degree Awarded: Dr. Nedaa Y. Ali Alsarraf
Keywords: Halophilic,Halotolerant Plant Growth , Rhizobacteria , (PGPR) from the Rhizosphere of Tamarix aucheriana in Kuwait
Issue Date: 2019
Publisher:  Kuwait university - college of graduate studies
Abstract: Soil salinity is amongst the strongest factors influencing plant growth and production. About 10% of Kuwait’s total land area is covered by Sabkha a form of salt flat. In the current study a total of 37 different halophilic and halotolerant bacterial strains were isolated from the rhizosphere of Tamarix aucheriana, a halophytic plant that grows in Sabkha in different areas in Kuwait Doha (14 strains), South area (Om Alhyman and Julayah) and North area (Jahra and Khazma) (23 strains). All isolates were characterized using 16S rRNA sequence analysis and grouped well into homogenous clades showing close relatedness between them and reflected their similar physiological properties and salt preferences. Doha isolates were characterized into five major bacterial genera: Bacillus, Halobacillus, Halomonas, Nesterenkonia, and Piscibacillus with differing sub-branches indicating the divergent species within the same genera. Similarly, the 23 halotolerant and halophilic bacterial isolates from South and North areas 16S rRNA analysis showed that they were distributed into seven major bacterial genera: Bacillus, Oceanobacillus, Gracilibacillus, Salinicoccus, Nestrenkonia, Kushnaria and Halomonas. All of these genera are known to be moderate halophiles. Both evolutionary analyses revealed mutual genera found in the different sites; Bacillus, Halomonas. and Nestrenkonia. Doha isolates were further studied using phenotypic, biochemical tests (API) and were screened for multiple PGPR traits (IAA production, phosphate-solubolization, fixed nitrogen-production and IAR test). The results indicated that all bacterial isolates were to perform different PGPR activities including indole acetic acid production, potential nitrogen fixation and displayed resistance to one or several antibiotics. Finally, a greenhouse experiment was conducted to test the potential PGPR activity for five of these Doha strains. The strains were inoculated in to the rhizosphere of tomato plants. All the selected strains showed no significant difference on the height of the plants. However, the effect on both fresh and dry weight was quite significant statistically.
Appears in Programs:0495 Microbiology

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