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Title: Immunological Analysis of Dental Pulp Inflammation
Authors: Mohamed Maged Elsalhy 
Supervisor: Prof. Raj Raghupathy
Keywords: inflammation;versus invasive dental treatment;Dental Pulp
Issue Date: 2011
Publisher:  Kuwait university - college of graduate studies
Abstract: Assessing the degree of inflammation in the dental pulp poses a diagnostic dilemma as it influences the decision between conservative versus invasive dental treatment. However, there are no objective, quantitative and clinically-practical methods for evaluating pulpal inflammation. Cytokines have been suggested to be markers of pulpal inflammation, but estimation of cytokine levels has been possible only after extraction of inflamed teeth. We set out to develop a method to measure cytokines from the dental pulp prior to decision making. One hundred and eight dental pulp blood samples were obtained with cotton pellets from pulp sites exposed on pulpectomy. Twenty five samples were from normal teeth, 40 from asymptomatic pulps with caries exposure and 43 from symptomatic pulps clinically diagnosed as irreversible pulpitis. The levels of the inflammatory cytokines IL-2, IL-6, IL-8, TNF-α and IFN-γ and the anti-inflammatory cytokine IL-10 were quantified using high-sensitivity ELISA. Levels of cytokines and ratios of inflammatory cytokines to IL-10 were compared using Kruskal–Wallis and Mann–Whitney tests. Significantly higher levels of IL-6, IL-8, IL-10, TNF-α and IFN-γ were detected in caries-exposed and irreversible pulpitis as compared to normal teeth. IL-2 levels were higher in caries-exposed as compared to normal teeth. IL-2 and IL-10 levels were higher in caries-exposed pulps as compared to irreversible pulpitis, while IL-8 was higher in irreversible pulpitis as compared to caries-exposed teeth. Most interestingly, IL-6/IL-10 and IL-8/IL-10 ratios were significantly higher in irreversible pulpitis compared to both caries-exposed and normal teeth. v IL-8 levels and the IL-8/IL-10 ratio promise to be good indicators of irreversible pulpitis. An important and potentially very useful outcome of this study is the demonstration that cytokine estimation in pulpal blood may help in the diagnosis of pulpal inflammation.
Appears in Programs:0520 Microbiology (M.Sc.)

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