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Title: Abundance and Diversity of Halophilic Archaea in Kuwait Tidal Flats
Authors: انوار سمير القطان 
Supervisor: د. هدى محمود
Keywords: archaeal diversity halophilic genera
Issue Date: 2014
Publisher:  Kuwait university - college of graduate studies
Abstract: Tidal flats are productive marine ecosystems that occupy a strategic logistic position as intermediate zones between land and sea, therefore, they are expected to harbor unique microbial groups. Among the least studied groups of microbes in the tidal flats are the Archaea. Thus; the current study focuses the attention to the archaeal community inhabiting tidal flats north of the Arabian Gulf. During the study, archaeal diversity and abundance was investigated in Kuwait at bay and non-bay stations during summer and autumn. Sediment samples from the tidal flats were collected using core samplers and the total number of archaea was determined using fluorescent in situ hybridization (FISH). The culturable and uncultured archaea were investigated using 7 types of media and PCR-DGGE-Sequencing technique respectively. The results showed that Haloferax sp., Halogeometricum sp. and Natrinema sp. dominated the culturable archaea while the Halorhabdus sp., Halomicrobium sp. and Halorussus sp. dominated the uncultured archaea. In addition, the FISH results showed that the total numbers of archaea were relatively higher in summer than in autumn and in bay-stations than in non-bay stations. In addition, the Archaea population decreased as the depth of the sediment increased, and muddy sediments harbored higher numbers of archaea than sandy sediments. In conclusion, the archaeal diversity in the uppermost surface of the tidal flats north of the Arabian Gulf is dominated by certain halophilic genera.
Appears in Programs:0495 Microbiology

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