Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/224
Title: Amplification of ESR1 Gene in Kuwaiti Breast Cancer Patients
Authors: ابرار عبدالرضا الحداد 
Supervisor: د. فهد الملا
Keywords: ESR1 Gene Kuwaiti Breast Cancer
Issue Date: 2011
Publisher:  Kuwait university - college of graduate studies
Abstract: iv ABSTRACT Around two-thirds of Kuwaiti women with breast cancer are estrogen receptor (ER)-positive. The reason behind this selection is not known. Estrogens, ERs, and their cellular responsiveness have been widely studied in humans, animal models, and cell lines. The two types of ERs, ER alpha (ERα) and beta (ERβ), may be involved in the resistance and aggressiveness of carcinomas. Thus, ERs are one of the most important therapeutic targets in breast cancer. Amplification of the ESR1 gene, which encodes ERα, at q25 of chromosome 6 has recently been reported to drive the expression of ERα in Caucasian breast cancer patients. Here, we sought to determine whether breast cancer in Kuwaiti citizens, which commonly express ERs, could be driven by the same type of gene amplification. Eighty-eight Kuwaiti breast cancer cases collected from the Kuwait Cancer Control Center were used in this study. Hematoxylin and eosin stained sections were used to determine tumor type. ERα protein production was determined using immune reactive score immunohistochemistry. ESR1 copy number was investigated using chromogenic in situ hybridization (CISH) and quantitative real time PCR (qPCR). We found that most (65.91%) of the histological tumor types collected were invasive ductal carcinoma. ERα protein was expressed in the majority of samples (69.33%). There were no ESR1 amplification events detected using CISH in the 88 breast cancer samples. However, qPCR applied for 25 breast cancer samples, identified one (4%) ESR1 amplification event and two (8%) gains.
URI: http://hdl.handle.net/123456789/224
Appears in Programs:2050 Molecular Biology

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