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|Title:||Detection and Genotyping Camel Rotaviruses in Kuwait||Authors:||ليلى ذعار المطيري||Supervisor:||د. جورج سودز||Keywords:||Camel Rotaviruses Kuwait||Issue Date:||2011||Publisher:||Kuwait university - college of graduate studies||Abstract:||iv Abstract Rotaviruses are the major cause of acute viral gastroenteritis in neonates and young children worldwide, and in the young animals of a large variety of animal species. Animal rotaviruses have a huge economic and zoonotic importance, as they are a potential reservoir for genetic exchange with human rotaviruses. Camels are economically important animals in the desert regions of North Africa and the Middle East, including Kuwait. Although human and bovine rotaviruses have been incriminated as causes of diarrhea in children and newborn calves in Kuwait, no data is available on the role of rotavirus in camel calves diarrhea. The objective of this study was to detect and characterize rotavirus in camels in Kuwait. Between 2008 and 2010, 408 fecal samples were collected from camels and tested by ELISA kits in five geographic areas; only one sample was found to be positive for rotavirus. However, 308 serum samples taken from camels revealed that 177 (57%) samples contained rotavirus antibodies. Serotesting of different age groups of camels for rotavirus-specific antibodies revealed that positive samples were increased after one year of age and by two years of age, all camels had rotavirus antibodies. Using VP6-specific RT-PCR, 8 rotavirus strains from 109 randomly selected fecal samples were found in 4 of 5 geographic areas. One of them, 21s/10, was genotyped as G10P and this strain is closely related to lamb rotaviruses. This G10 camel rotavirus strain is believed to be the third isolate from camels, and it is the first camel rotavirus with G10P characteristics observed to date. Further studies are needed to collect more detailed and extensive data for the presence of this unique rotavirus genotype and its possible interspecies transmission.||URI:||http://hdl.handle.net/123456789/226|
|Appears in Programs:||2050 Molecular Biology|
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