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Title: Bioactivity Guided Fractionation to Isolate Anticancer Lead(s) from Salvia aegyptiaca L. and Thymus vulgaris L.: Potential Molecular Mechanisms Of Action
Authors: Ebtehal Yousef Al-Qattan 
Supervisor: Dr. Mohamed S. Abaza
Keywords: Anticancer Lead
Issue Date: 2013
Publisher:  Kuwait university - college of graduate studies
Abstract: Cancer is a devastating disease that causes death to millions of people every year. Thus intense research had been made on several natural sources to develop novel anticancer drugs. In the present study, two plants, Salvia aegyptiaca (Labiatae) and Thymus vulgaris (Lamiaceae) were, separately, extracted in 96% ethanol then subjected to solvent/solvent partitioning processes and purification techniques. The identities of the isolated compounds were identified using different spectral techniques. Salvia aegyptiaca yielded the known sterol, β-sitosterol, while Thymus vulgaris gave naringenin (Nar) and aromadendrin (Aro). Nar and Aro showed time- and dose-dependent anti-proliferative effects on human colorectal, breast and melanoma cancer cell lines. Exposure to Nar resulted in accumulation of human breast and colorectal cancer cell lines in the S-phase and G2/M-phase as well as melanoma cell lines in the S-phase. Nar induced apoptosis in colorectal CCL233 (56.4% vs. 2.5% for UT), breast HTB26 (95.2% vs. 2.4% for UT), and melanoma HTB66 (81.7% vs. 4.2% for UT) cancer cell lines. Similar results were obtained with CCL235, HTB132 and HTB68 cancer cell lines. Nar differentially down-regulated the expression of Cdk4, Cdk6, Cdk7, Bcl2, x-IAP and c-IAP-2, differentially up-regulated the expression of p18, p19, p21, caspases 3, 7, 8 and 9, Bak, AIF and Bax in both human colorectal and breast cancer cell lines. In conclusion, Nar and Aro may be potential agents for prevention and/or treatment of human cancers. However, further studies should be conducted in appropriate animal models of cancer, and ultimately, human cancer prevention trials should be performed.
Appears in Programs:2050 Molecular Biology

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