Please use this identifier to cite or link to this item:
Title: Lead Burden in the Body: Effects on Heart Tolerance to Ischemia-Reperfusion Injury and its Response to Postconditioning Protection
Authors: Aisha Jassim Al-Kouh 
Supervisor: Dr. Fawzi Babiker
Keywords: Lead Burden Heart Ischemia-Reperfusion Postconditioning
Issue Date: 2015
Publisher:  Kuwait university - college of graduate studies
Abstract: Cardiovascular diseases (CVDs) are a leading cause of morbidity and mortality in the world in general, and the Middle East in particular. The influence of lead toxicity on the heart and the prognosis of CVDs are seldom investigated. In this study we investigated 1) the effect of lead (Pb) toxicity on the structure of the heart, 2) its effect on the sensitivity of the heart to the ischemic insult, and 3) its impact on the protection of the heart against I/R by pacing post-conditioning (PPC). Adult male and female Wistar rats were subjected to 0.5% lead acetate in drinking water for 5 or 45 days. Hearts were isolated from these rats to study the effect of Pb toxicity structure and the changes that may lead to the expression of fetal genes using immunohistochemistry. Changes in oxidants, antioxidants and apoptotic cell death were assessed using standard techniques. Langendorff system was used for the evaluation of heart functions. Lead exposure resulted in a significant (P<0.001) decrease in body weight of male and female rats and increased heart weight in male rats. Long-term exposure to Pb significantly (P<0.01) increased the expression of Ki-67, c-Myc, c-Jun and increased apoptotic cell death in the heart as compared to respective controls. However, Lead exposure did not affect the total antioxidant level but affected the total oxidant level significantly (P<0.001) as compared to controls. Moreover, Pb exposure did not affect the heart sensitivity to ischemic insults; it almost completely abrogated PPC protection to the heart against I/R injury. Interestingly, this lack of protection is associated with a significant (P<0.001) decrease in the total anti-oxidant levels as compared to control. In contrast, the protection demonstrated in the short-term Pb-treated heart was associated with a significant (P<0.001) decrease in the total oxidant level compared to control. Lead toxicity caused a dramatic increase in the heart weights in male rats, increased fetal gene expression, apoptosis and antioxidants’ level. Long-term lead exposure eliminated PPC protection to the heart against I/R injury.
Appears in Programs:0530 Physiology (M.Sc.)

Files in This Item:
File Description SizeFormat 
Final May 2015 Aisha thesis YM02-13.pdf3,26 MBAdobe PDFView/Open    Request a copy
Show full item record

Page view(s) 50

Last Week
Last month
checked on Jul 16, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.