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Title: Role of (-)-Epigallocatechin-3-gallate on Hippocampal Neurogenesis in Diabetic Rats and Cultured Hippocampal Cells in In vitro Hyperglycemic Model of Diabetes
Authors: Sayedah Fareedah Ali Al-Mohri 
Supervisor: Dr. Muddanna S Rao
Keywords: Epigallocatechin-3-gallate Hippocampal Neurogenesis in Diabetic Rats Hippocampal Cells
Issue Date: 2015
Publisher:  Kuwait university - college of graduate studies
Abstract: Diabetes mellitus is a chronic endocrine disease characterized by high level of glucose in blood. Studies with diabetic rats have shown neuronal apoptosis, decreased dendritic complexity and compromised learning and memory. Increased glucose concentration in the culture media itself is shown to be detrimental to neurons and astrocytes. Among many types of catechins in green tea, (-)-Epigallocatechin-3-gallate (EGCG) comprising about one-third of green tea dry mass, passes through the blood–brain barrier to reach the brain parenchyma, and is primarily responsible for its beneficial effects. Although literature indicated the beneficial role of EGCG on hippocampal neurogenesis, there are no studies on its role in neurogenesis in vivo diabetic model and in vitro neural culture. Objectives of the present series of experiments was to study the effects of EGCG on learning and memory, adult neurogenesis in streptozotocin (STZ) model of diabetes in young rats and cultured hippocampal cells (neurons and astrocytes) in vitro hyperglycemic model of diabetes. Wistar rats (3 month old) were divided into i) Vehicle control (VC) - treated with a single injection of citrate buffer, ii) EGCG - treated daily with EGCG (50mg/kg, ip), iii) Diabetic (DI) - Diabetes was induced with STZ (40mg/kg ip), iv) Diabetic + EGCG treated (DI+EGCG) - Diabetes was induced with STZ and treated with EGCG (50mg/kg, ip). In in vitro experiment, 7 days old primary cultures of fetal hippocampal tissue were divided into i) Control culture (C) - no treatment, ii) (EGCG) - treated with EGCG (50μM), iii) Hyperglycemic culture (HG) - media contained 75mM glucose and iv) HG + EGCG - media contained 75mM glucose and treated with EGCG (50μM). Results of in vivo experiments showed memory enhancement, increased neurogenesis, increased cell proliferation, decreased degenerative changes in diabetic rats treated with EGCG compared to DI groups. In vitro experiment revealed enhanced neuronal survival in cultures treated with EGCG. These results suggest that EGCG protects the neurons from degeneration in diabetic hippocampus and high glucose condition in culture. Probable mechanisms may be related to anti-inflammatory, neurogenesis -and gliogenesis- inducing properties of EGCG. We conclude that EGCG has beneficial role in minimizing the effects of diabetes in the hippocampus.
Appears in Programs:0560 Anatomy

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