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Title: Antimicrobial Activity of Novel Metalloporphyrin-based Photosensitizers
Authors: سارة صالح مغنية 
Supervisor: د. ليدميل بينوف
Keywords: Antimicrobial Activity Photosensitizers
Issue Date: 2014
Publisher:  Kuwait university - college of graduate studies
Abstract: The finding that some dyes (generally porphyrins) are taken up and show preferential accumulation in tumors inspired the development of photodynamic therapy (PDT) in clinical practice. PDT is a non-invasive treatment option, based on the use of a light-absorbing compound (photosensitizer, PS) and irradiation with visible light. In the presence of molecular oxygen, light activation of the PS leads to the local production of singlet oxygen and other reactive oxygen species (ROS) that damage and kill the target cells. Since the properties of a PS are crucial for the efficacy of PDT, in recent years research activity has been directed towards synthesis and characterization of new PSs. Cationic Zn(II)N-alkylpyridylporphyrins (ZnPs) with amphipathic properties are potent photosensitizers (PSs) capable of killing antibiotic-resistant pathogenic bacteria and tumor cells. Mycotic infections of humans and animals are widespread and difficult to treat. Rising resistance against current antifungal medications has stimulated the exploration of alternative treatment modalities. Antimicrobial PDT is a potential candidate because it can kill a wide range of microorganisms, including resistant yeasts, bacteria, and viruses. The aim of this study is to determine the antifungal activities and photodynamic efficiency of newly synthesized metalloporphyrins and compare these with commercially available PSs. The antimicrobial photodynamic efficiency of PSs was assessed by investigating their effect on yeast cell viability using MTT assay. Yeast suspensions (S. cerevisiae) were grown to A600~ 0.1. Aliquots of 100 μl were incubated with different concentrations of PSs for 90 minutes and illuminated for 60 min. Cell viability was then determined. Results showed that the antifungal PDI efficiency of ZnPs increased as their lipophilicity increased; the long-chain amphiphilic ZnPs were more efficient than the more hydrophilic short-chain analogs. Increasing the iv hydrophobicity of ZnP molecules increases their cellular accumulation. On the other hand, the hydrophobic PS Chlorin e6, at 5 and 10 μM, demonstrated lower photoefficiency than the ZnP hexyl derivative. These results indicate that the amphiphilic hexyl-substituted ZnP is a promising PS for antifungal photodynamic therapy.
Appears in Programs:0540 Medical Biochemistry (M.Sc.)

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