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Title: Characterization of Klebsiella pneumoniae Isolated from Kuwait Hospitals
Authors: Hassan Ali Ghloum Haider 
Supervisor: Dr. Esmaeil Al-Saleh
Keywords: Klebsiella pneumoniae , Kuwait Hospitals
Issue Date: 2016
Publisher:  Kuwait university - college of graduate studies
Abstract: The management of the medically important infections caused by the highly pathogenic Klebsiella pneumoniae strains is usually initiated by the characterization of the causative agents. The current study characterized Klebsiella pneumoniae isolated from urine samples collected from UTI patients in Kuwait general hospitals. The majority of isolates were identified as Klebsiella pneumoniae (39.4%) causing UTI to all members of the Kuwaiti population in particular to females aged between 41 and 70 years old. The molecular and biochemical analyses clustered the isolates into 5 phylogroups and 6 metabotypes, respectively, which reflected the highly diverse nature of the isolates. Determination of the antibiotic susceptibility profiles showed that 43% of isolated Klebsiella pneumoniae in Kuwait were ESBL producers able to resist highly effective antibiotics such as members of the third generation cephalosporins and carbapenes. In addition, the genes coding for ESBL production in the isolated strains were characterized. The highest occurrence was shown for bla-TEM gene (90%). On the other hand, the lowest incidence was demonstrated for OXA and AmpC genes (5%). Also, KPC gene was detected in 15% of the ESBL producing isolates. To our knowledge, the current study is considered the first nationwide investigation about the prevalence of KPC gene among Klebsiella pneumoniae strains in Kuwait hospitals. The determination of the antibiotic susceptibility testing showed that the majority of tested strains was sensitive to some available antibiotics such as colistin, imipenem and meropenem. Moreover, isolated strains were shown to harbor generally the similar factors such as biofilm formation, production of surface active compounds and elastase activity that enable Klebsiella pneumoniae to inevitably invade, transmit and infect all members of Kuwait population.
Appears in Programs:2040 Environmental Sciences

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