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Title: Qualitative and Quantitative PCR Analyses of Genetically Modified Food and Feed Products from Kuwait Market
Authors: Dalal Razkan Alreshidi 
Supervisor: Dr. Suad S. Almazrooei
Keywords: certified reference material : cycle threshold
Issue Date: 2017
Publisher:  Kuwait university - college of graduate studies
Abstract: Agricultural plants are being the most frequently genetically modified organisms (GMOs) because of their large contribution in livelihood as well as sources of food in many countries globally. The genome of many crops has been modified and new varieties of transgenic plants have been produced. These new varieties possess novel genes that impart beneficial characteristics and traits. On the other hand, the rapid development of genetically modified (GM) crops has become a matter of concern among the public and consumers. Most countries in the Middle East, including Kuwait, do not have a routine certified system to regulate the import of GM crops. In this study, 36 samples of food and feed products of corn, soybean and rice sold in local markets were screened for the presence of modified genes using both qualitative and quantitative polymerase chain reaction (PCR)-based approaches. Among the different DNA extraction methods that were evaluated, a modified CTAB isolation protocol was adopted which resulted in higher quantity and better quality of DNA. Conventional PCR was used first to identify potential GM products by alternately amplifying the sequences of cauliflower mosaic virus 35S promoter (p35S) and the Agrobacterium nopaline synthase terminator (Tnos). Suspected positive samples were further confirmed by quantitative real-time PCR (qPCR). Results demonstrated the presence of GM sequences in 6 out of 26, 1 out of 5 and 2 out of 5 samples of corn, soybean and rice respectively. Furthermore, droplet digital PCR (ddPCR) test revealed that almost all GM-derived corn samples contained the GM event “MON810” with an amount ranges between 3.50% and 22.30% m/m which exceeded the accepted international threshold level. This study reports for the first time the detection of GMOs in animal feed as well as the use of ddPCR for GMOs quantification in food/feed products from Kuwaiti local markets.
Appears in Programs:0494 Plant Biology

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