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Title: Plasmid Mediated Quinolone Resistance in Escherichia coli O25 ST131 from Kuwait
Authors: Eman Jasem Al-Sarraf 
Supervisor: Prof. Ali Dashti
Keywords: base pair : kanamycin
Issue Date: 2017
Publisher:  Kuwait university - college of graduate studies
Abstract: Escherichia coli O25 ST131 is commonly found in urine samples of patients with urinary tract infections and has the potential to become multi- drug resistant. Eight epidemic isolates were obtained from Mubarak Hospital Intensive care Unit (ICU) and were used for this research. The sequence type of these isolates were determined by performing a polymerase chain reaction for the pabB gene. Antimicrobial susceptibility testing was done by disc diffusion method for ten antimicrobial agents and the results were interpreted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI).In addition, E-test and Minimum Inhibitory Concentration (MIC) were determined. Polymerase chain reaction (PCR) was performed for (blaTEM, blaSHV & blaCTX-M), tetracycline resistance genes (tetA & tetB), fluoroquinolone resistance genes (qnrA, qnrB, qnrS, parC, aac(6')-cr-lb and gyrA) and colistin resistance genes (mcr-1 and pmrB). Pulse fieldgel electrophoresis (PFGE) was used to determine the relatedness between the isolates. The results showed that 87.5% of the isolates were resistant to ciprofloxacin, piperacillin and trimethoprim/sulfamethazole. 75% of the isolates were resistant to gentamycin and only 25% were resistant to chloramphenicol. On the other hand 100% of the isolates were resistant to the other used antibiotics (amoxicillin/clavulanic acid, ampicillin/sulbaclam, ceftazidime, kanamycin and tetracyline). Minimum inhibitory concentration of colistin was 4 mg/L for five isolates. The MIC was 2 mg/L for two isolates. Isolate c-55 was colistin resistant with MIC > 128 mg/L. blaTEM was the most detected gene among the ESBLs which was found in 87.5% of the isolates. Regarding tetracycline resistance genes, tetA was the most detected (37.5%). Interestingly we detected the pmrB gene in 6 isolates (75%). PFGE showed 100% similarity of 3 isolates (c-14, c-26 and c-30). The other fine isolates were diverse. The epidemic Escherichia coli O25 ST131 strains processed in this research although small in number were resistant to antibiotics including colistin which makes the treatment of these infections increasingly challenging.
Appears in Programs:0712 Medical Laboratory Sciences

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