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Title: Neurotherapeutic Effects of Ginkgo Biloba Extract and Its Isolated Ginkgolide B
Authors: Dalal Ghunaim Aladwani 
Supervisor: Dr. Khaled Y. Orabi
Keywords: Neurotherapeutic
Issue Date: 2018
Publisher:  Kuwait university - college of graduate studies
Abstract: Ginkgo biloba L is an old Chinese tree. Its extract (GBE) has been traditionally used to treat many diseases such as Alzheimer’s disease, age-associated dementia, cerebral insufficiency and schizophrenia. This study describes the extraction, purification and neuroprotective effect evaluation of ginkgolide B (G-B) in the crush sciatic nerve injury rat model. Two experiments were carried out during two sequential periods; fractionation and isolation, and randomized, double-blinded in vivo neuroanatomical attributions. Fractionation and isolation of G-B was accomplished by using vacuum liquid chromatography and prep-HPLC. The isolated G-B was identified using different spectral techniques. In addition, terpene trilactones-enriched extract (TEGBE) was prepared. To evaluate the neuroprotective effects, sixty Wistar male rats were randomly allocated into 6 groups: NAÏVE (n=6), SHAM (n=12), CRUSH+ normal saline (n=12), CRUSH+GBE (n=6, 50 mg/kg, intraperitoneally (i.p)), CRUSH+TEGBE (n=12, 50 mg/kg, i.p), and CRUSH+G-B (n=12, 10 mg/kg i.p). Treatments were given one hour following injury and daily for 14 days. Neurobehavioral tests, histomorphological examinations, and immunohistochemistry analysis of sciatic nerve and spinal cord were performed at weeks 3 and 6 post injury. Results revealed that GBE, TEGBE and G-B enhanced functional and sensory behavioral parameters and protected the ultrastructural elements in the sciatic nerve. Also, they prevented spinal cord neurons from further deterioration following sciatic nerve injury. In conclusion, GBE and its isolated G-B exhibit positive neurotherapeutic effects on crush sciatic nerve injury model, G-B treatment showed the most neuroprotective outcome compared to other constituents. This study paves the way for more in-depth analysis of the mechanisms through which G. biloba constituents are involved in nerve regeneration and neuronal protection following injury.
Appears in Programs:1100 Pharmaceutical Sciences

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