Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/769
Title: Isolation of Anaerobes, Specifically Prevotella Species, From Patients with Oro-Dental Infections
Authors: Ameerah Mahdi Al Azemi 
Supervisor: Dr. Wafaa Jamal
Keywords: Isolation of Anaerobes
Issue Date: 2018
Publisher:  Kuwait university - college of graduate studies
Abstract: iv ABSTRACT Periodontal diseases are a group of chronic infections that destroy tissues surrounding and supporting the teeth. There is no data on the anaerobes associated with periodontal infections in Kuwait. The objective of this study was to investigate the target anaerobes associated with periodontal diseases in Kuwait. Patients with chronic periodontitis, seen at the Kuwait University Dental Clinics, were recruited into this study during a period of 15 months. Samples were collected using Gutta Percha Points directly from inside the gingival pockets and transported in sterile Ringer’s solution and pre-reduced thioglycolate broth to the Laboratory. One set of samples was cultured on appropriate media and the other set was used for PCR assays. DNA sequencing and semi-quantitative PCR were performed for Prevotella spp. only. A total of 30 patients stratified according to the severity of the disease; mild (3 patients), moderate (14), and severe (13), were studied. Thirty-one healthy individuals were included as controls. Thirty percent of the patients were between 50-59 years age group. Female-to-male ratio was 1:2. The ratio of Kuwaitis to non-Kuwaitis was 1:3.3. Aggregatibacter actinomycetemcomitans were found in 23.3% versus 16.1% of cases and controls, respectively (P>0.05). All the 30 (100%) patients harbored Fusobacterium spp. compared to 93.5% of the controls(P>0.05). Porphyromonas gingivalis was detected in 33.3% of patients versus 6.4% of the controls (P<0.0001). Tannerella forsythia was detected in 83.3% patients versus 51.6% controls (P<0.0001). Parvimonas micra was detected in 90% patients versus 51.6% controls (P<0.0001) and Treponema denticola was detected in 70% patients versus 29% controls (P<0.0001). Prevotella spp. were detected in all the patients and controls; the majority was P. nigrescens detected in 70% and 100% of patients and controls. There was no significant difference in DNA concentrations of Prevotella spp. between patients and controls by semi-quantitative PCR assay. In conclusion, all the target anaerobes, except Aggregatibacter spp., Prevotella spp. and Fusobacterium spp., were significantly associated with periodontitis in our study. P. gingivalis was the most strongly associated anaerobe with periodontal disease, while Prevotella spp. appear to be normal passengers.
URI: http://hdl.handle.net/123456789/769
Appears in Programs:0520 Microbiology (M.Sc.)

Files in This Item:
File Description SizeFormat 
Ameerah Alazemi march 2018.pdf1,37 MBAdobe PDFView/Open    Request a copy
Show full item record

Page view(s)

33
Last Week
0
Last month
1
checked on Sep 21, 2020

Download(s)

8
checked on Sep 21, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.