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Title: Chronic Hydrogen Sulfide Donor Alters Microglial Activation in the Spinal Cord of Streptozotocin-induced Diabetic Rats
Authors: AbdulAziz M. F. Shayea 
Supervisor: Dr. Alyaa Mousa
Keywords: Chronic Hydrogen
Issue Date: 2018
Publisher:  Kuwait university - college of graduate studies
Abstract: long-term diabetic patients suffer immensely from diabetic neuropathy. The present study was designed to investigate the effects of Hydrogen sulfide (H2S) on peripheral neuropathy, activation of microglia and the cascade secretion of pro-inflammatory cytokines, such as interleukin-1beta (IL-1β), interleukin- 6 (IL-6), Tumor necrosis factor (TNFα), in the streptozotocin-induced peripheral diabetic neuropathy rat model. Male Sprague-Dawley Rats (SD) were injected intraperitoneally (ip) with streptozotocin )STZ( (55 mg STZ/kg body) to induce type 1 diabetes mellitus. Diabetic-induced rats were treated with water-soluble, slow-releasing H2S donor GYY 4137 (50 mg/kg) daily i.p. for four weeks to deliver exogenous H2S to the spinal cord. The effect of multiple doses of H2S on rat’s antiallodynic/antihyperalgesic activities was evaluated by Von Fery Test, Paw pressure test, and Hot Plate. Moreover, the histopathology of the spinal cord examined and the effect of H2S on the spinal expression of microglial proinflammatory cytokines analyzed with Western blot. The present study showed neuroprotective effects of H2S on the spinal cord of diabetic animals and modulated their sensory deficits that tested with neurobehavioral tests. H2S treatment decreases allodynia (p<0.05) and mechanical hyperalgesia (p<0.01) in comparison to diabetic rats and increases thermal hyperalgesia (p<0.00) compare to diabetic rats. H2S treatment decreases microglia in both grey and white matter of the spinal cord compared to diabetes. Also, proinflammatory cytokines levels were reduced in treated rats compared to diabetic rats. H2S has a potential ameliorative effect on the neuropathic pain through the control of microglial activation and microglia-mediated inflammation that may be considered in the future as a possible treatment of peripheral nerve sensation in diabetic patients
Appears in Programs:0560 Anatomy

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